PXD052786-1

PXD052786 is an original dataset announced via ProteomeXchange.

Title	Genome dilution by cell growth drives starvation-like proteome remodeling in yeast and mammalian cells
Description	Cell size is tightly controlled in healthy tissues and single-celled organisms, but it remains unclear how cell size influences physiology. Increasing cell size was recently shown to remodel the proteomes of cultured human cells, demonstrating that large and small cells of the same type can be compositionally different. Here, we utilize the natural heterogeneity of hepatocyte ploidy and yeast genetics to establish that ploidy-to-cell size ratio is a highly conserved determinant of proteome composition. In both mammalian and yeast cells, genome dilution by cell growth elicits a starvation-like phenotype, suggesting that growth in large cells is restricted by genome concentration in manner that mimics a limiting nutrient. Moreover, genome dilution explains some proteomic changes ascribed to yeast aging. Overall, our data indicate that genome concentration drives changes in cell composition independently of external environmental cues.
HostingRepository	PRIDE
AnnounceDate	2024-06-05
AnnouncementXML	Submission_2024-06-05_12:57:00.686.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Lanz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-02 22:11:27	ID requested
⏵ 1	2024-06-05 12:57:01	announced
2	2024-10-22 06:43:49	announced	2024-10-22: Updated project metadata.

Lanz MC, Zhang S, Swaffer MP, Hern, á, ndez G, ö, tz L, McCarty F, Ziv I, Jarosz DF, Elias JE, Skotheim JM, Genome dilution by cell growth drives starvation-like proteome remodeling in mammalian and yeast cells. bioRxiv, ():(2023) [pubmed]

10.1101/2023.10.16.562558;

submitter keyword: Yeast, TMT, Human, SILAC

Jan Skotheim
contact affiliation	Stanford University
contact email	skotheim@stanford.edu
lab head
Michael Lanz
contact affiliation	Stanford University
contact email	mikelanz@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD052786

PRIDE project URI

• PRIDE
  1. PXD052786
     1. Label: PRIDE project
     2. Name: Genome dilution by cell growth drives starvation-like proteome remodeling in yeast and mammalian cells