PXD052756-1

PXD052756 is an original dataset announced via ProteomeXchange.

Title	LC-MS Thermal proteome profiling in Arabidopsis thaliana to study ascorbate interaction
Description	Ascorbate is a major plant metabolite that plays crucial roles in various processes, from reactive oxygen scavenging to epigenetic regulation. However, to what extent and how ascorbate modulates metabolism is largely unknown. To address this, we investigated the consequences of chloroplastic and total cellular ascorbate-deficiencies by studying chloroplastic ascorbate transporter pht4;4 mutant lines, and the ascorbate-deficient vtc2-4 mutant of Arabidopsis thaliana. Under regular growth conditions, both ascorbate-deficiencies caused minor alterations in photosynthesis, with no apparent signs of oxidative damage. In contrast, metabolomics analysis revealed a global and largely overlapping metabolome rewiring in both ascorbate deficiencies, suggesting that chloroplastic ascorbate modulates plant metabolism. We observed significant alterations in amino acid metabolism, particularly in arginine metabolism, activation of nucleotide salvage pathways, and changes in secondary metabolism. In addition, proteome wide analysis of thermostability revealed that ascorbate may interact with enzymes involved in arginine metabolism, the Calvin-Benson cycle, and several photosynthetic electron transport components. Overall, our results suggest that, independently of oxidative stress, chloroplastic ascorbate interconnects and coordinates diverse metabolic pathways in vascular plants and thus acts as a regulatory hub.
HostingRepository	PRIDE
AnnounceDate	2024-08-23
AnnouncementXML	Submission_2024-08-23_01:24:25.026.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052756
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Anna Karlsson
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-31 06:45:36	ID requested
⏵ 1	2024-08-23 01:24:25	announced
2	2024-10-22 06:56:06	announced	2024-10-22: Updated project metadata.

10.1093/PLPHYS/KIAE409;

10.6019/PXD052756;

submitter keyword: Arabidopsis thaliana, termal proteome profiling, Q-Exactive HF Hybrid Quadrupole-Orbitrap

Anna Karlsson
contact affiliation	KTH Royal Institute of Technology, Sweden
contact email	annaka7@kth.se
lab head
Anna Karlsson
contact affiliation	KTH Royal Institute of Technonogy
contact email	annaka7@kth.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052756
     1. Label: PRIDE project
     2. Name: LC-MS Thermal proteome profiling in Arabidopsis thaliana to study ascorbate interaction