PXD052753-1

PXD052753 is an original dataset announced via ProteomeXchange.

Title	Comparative quantitative proteomic analysis of Chlamydomonas reinhardtii cells from wild-type and chimeric FNR-PSAF mutant cells
Description	Photosynthetic linear electron transfer (LET) comprises a solar energy driven pathway tha directs electrons to the production of NADPH, via ferredoxin-NADP+ reductase (FNR), while generating a proton motive force (pmf) to drive ATP synthesis. Both NADPH and ATP are consumed for carbon fixation. However, this pathway can be altered to block NADPH formation while maintaining pmf to produce ATP to enable acclimation of the organism to stress conditions at the expense of carbon fixation. LET and the alternative pathway, cyclic electron transfer (CET) both rely on protein-protein interactions with FNR but creating a knock-out mutant to explore its contribution to LET/CET regulation would be problematic given that FNR is essential. Instead, this project employs two mutants expressing a chimeric FNR-PSAF protein created by CRISPR-Cas9 gene editing: (1) NT12 = fnr-psaf/fnr/Δpsaf and (2) T7 = fnr-psaf/Δfnr/Δpsaf. The effects of these mutants, compared to wild-type cells, on the abundance of selected photosynthesis-related proteins are determined by MS-based quantitative proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_18:18:47.159.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-31 05:42:03	ID requested
⏵ 1	2025-05-06 18:18:48	announced

10.1093/plcell/koaf042;

Emrich-Mills TZ, Proctor MS, Degen GE, Jackson PJ, Richardson KH, Hawkings FR, Buchert F, Hitchcock A, Hunter CN, Mackinder LCM, Hippler M, Johnson MP, Tethering ferredoxin-NADP+ reductase to photosystem I promotes photosynthetic cyclic electron transfer. Plant Cell, 37(3):(2025) [pubmed]

submitter keyword: Chloroplast, Cyclic electron transfer, CRISPR-Cas9, Photosystem (PS), LET, Ferredoxin-NADP+ reductase,Chlamydomonas reinhardtii, PSI subunit PSAF, Linear electron transfer, CET

Professor Matthew Johnson
contact affiliation	Plants, Photosynthesis and Soil, School of Biosciences, University of Sheffield, UK
contact email	matt.johnson@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052753

PRIDE project URI

• PRIDE
  1. PXD052753
     1. Label: PRIDE project
     2. Name: Comparative quantitative proteomic analysis of Chlamydomonas reinhardtii cells from wild-type and chimeric FNR-PSAF mutant cells