PXD052743-1

PXD052743 is an original dataset announced via ProteomeXchange.

Title	Osteopontin Regulation of MerTK+ Macrophages Promotes Crohn’s Disease Intestinal Fibrosis
Description	The pathogenesis of intestinal fibrosis in Crohn’s disease (CD) remains unclear. Mer receptor tyrosine kinase (MerTK) is an immunosuppressive protein specifically expressed in macrophages. Osteopontin (OPN), also known as secreted phosphoprotein 1, contributes to inflammation and wound repair. This study investigates the potential profibrotic pathway in MerTK+ macrophages in order to provide a possible therapeutic target for intestinal fibrosis. MerTK expression in the inflamed and stenotic bowels was evaluated. The MerTK/ERK/TGF-β1 pathway was overactivated in the fibrotic intestinal tissues of patients with CD. This pathway was induced by epithelial cell apoptosis, resulting in activated fibroblasts with increased TGF-β1 secretion. OPN upregulated TGF production by altering ERK1/2 phosphorylation, as evidenced by OPN or MerTK knockdown and OPN overexpression in vitro. MerTK inhibitor UNC2025 alleviated intestinal fibrosis in mouse colitis models, suggesting a potential therapeutic target for intestinal fibrosis in patients with CD.
HostingRepository	iProX
AnnounceDate	2024-05-31
AnnouncementXML	Submission_2024-12-10_19:46:33.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juanhan Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-30 20:39:17	ID requested
⏵ 1	2024-12-10 19:46:35	announced

Liu J, Gong W, Liu P, Li Y, Jiang H, Wu C, Wu X, Zhao Y, Ren J, macrophages promotes Crohn's disease intestinal fibrosis. iScience, 27(7):110226(2024) [pubmed]

submitter keyword: Crohn's disease, Macrophage, MerTK, Intestine

Jianan Ren
contact affiliation	Nanjing University
contact email	jiananr@nju.edu.cn
lab head
Juanhan Liu
contact affiliation	Nanjing University
contact email	1558940856@qq.com
dataset submitter

iProX dataset URI