PXD052713-1

PXD052713 is an original dataset announced via ProteomeXchange.

Title	The histogenetic origin of malignant cells predicts their cyto-toxicity towards synthetic lethality utilizing the TK.007 system
Description	The efficacy of killing human cancer cells by a modified Herpes-simplex-virus thymidine kinase TK.007/ganciclovir (GCV) system was investigated in malignant cells of different histogenetic origin. The aim was to determine whether different histogenetic origins of cancer cells in them-selves influence their reaction towards an approach of synthetic lethality, which theoretically should be applicable independently of the cell type. Fifteen malignant human cell lines were transduced with a lentiviral vector to stably express the TK.007 gene and cell proliferation assays under GCV were performed. Among TK.007-expressing cell lines, lymphoma and leuke-mia cells were more susceptible to killing than solid cancer cells, while osteosarcoma and mela-noma cells exhibited an intermediate susceptibility. Similar differences in killing were also noted in wild-type non-transduced cells. This study highlights that the histogenetic origin of malignant cells strongly influences their susceptibility towards cytotoxic agents, with leukemias and lym-phomas being more sensitive than solid cancer cells.
HostingRepository	PRIDE
AnnounceDate	2025-01-18
AnnouncementXML	Submission_2025-01-17_17:34:36.416.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Mair
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-30 06:12:58	ID requested
⏵ 1	2025-01-17 17:34:37	announced

Wasserman JS, Faezov B, Patel KR, Kurimchak AM, Palacio SM, Glass DJ, Fowle H, McEwan BC, Xu Q, Zhao Z, Cressey L, Johnson N, Duncan JS, Kettenbach AN, Dunbrack RL, Gra, ñ, a X, /PP2A through helical motifs. Nat Commun, 15(1):5776(2024) [pubmed]

10.1038/s41467-024-50015-7;

submitter keyword: Chemotherapy

Drug Resistance

Suicide Gene Transduction

Herpes Simplex Virus Thymidine Kinase

Ganciclovir

Functional kinome profiling

Udo Schumacher
contact affiliation	Institute of Anatomy and Experimental Morphology, Center for Experimental Medicine, University Cancer Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
contact email	u.schumacher@uke.de
lab head
Thomas Mair
contact affiliation	Section for Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf
contact email	t.mair@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD052713

PRIDE project URI

• PRIDE
  1. PXD052713
     1. Label: PRIDE project
     2. Name: The histogenetic origin of malignant cells predicts their cyto-toxicity towards synthetic lethality utilizing the TK.007 system