PXD052684-1

PXD052684 is an original dataset announced via ProteomeXchange.

Title	PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response
Description	Pseudouridine (Ψ) is an abundant post-transcriptional modification found across all classes of RNA. Since its discovery in mRNAs a decade ago, it has been widely speculated that Y might govern additional post-transcriptional regulation of gene expression. Here, we demonstrate that one of the principal enzymes responsible for adding Y to mRNAs, pseudouridine synthase 7 (PUS7), accumulates in the cytoplasm under a variety of stress conditions in Saccharomyces cerevisiae and BEAS-2B human epithelial lung cells. The localization of PUS7 to the cytoplasm promotes Y-incorporation into hundreds of different mRNA sequences and increases cellular fitness under ROS and divalent metal ion stress. The preponderance of newly identified Y-sites lies within portions of the mRNA important for post-transcriptional control—-coding regions and 3’ UTRs. Quantitative proteomics reveal that shifts in the cellular post-transcriptional modification landscape upon PUS7 relocalization reshapes the proteome. Our data suggest a mechanism whereby stressors localize PUS7 in the cytoplasm to enable the direct modification and regulation of stress response mRNAs, thereby protecting cells from further stress-induced damage. (2A-PUS7-control 68283 2B-PUS7-control 68284 2C-PUS7-control 68285 4A-PUS7-NES-Co 68286 4A-PUS7-NES-Co 68287 4B-PUS7-NES-Co 68288 5A-PUS7-control-Co 68289 5B-PUS7-control-Co 68290 5C-PUS7-control-Co 68291 PUS7-NES_CU_1A 75059 PUS7-NES_CU_1B 75060 PUS7-NES_CU_1C 75061 PUS7-NLS_CU_2A 75062 PUS7-NLS_CU_2B 75063 PUS7-NLS_CU_2C 75064 PUS7-WT_CU_3A 75065 PUS7-WT_CU_3B 75066 PUS7-WT_CU_3C 75067)
HostingRepository	PRIDE
AnnounceDate	2026-01-28
AnnouncementXML	Submission_2026-01-28_01:51:00.758.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minli Ruan
SpeciesList	scientific name: Saccharomyces cerevisiae BY4741; NCBI TaxID: NEWT:1247190;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-29 14:30:23	ID requested
⏵ 1	2026-01-28 01:51:01	announced

Dataset with its publication pending

submitter keyword: None

Kristin Koutmou
contact affiliation	Associate Professor of Chemistry Dow Early Career Professor of Chemistry Associate Director of the Program in Chemical Biology Co-Director the Chemical Biology Interface Training Program University of Michigan 930 N University Ann Arbor, MI 48109
contact email	kkoutmou@umich.edu
lab head
Minli Ruan
contact affiliation	University of Michigan
contact email	mlruan@umich.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052684
     1. Label: PRIDE project
     2. Name: PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response