PXD052612-1

PXD052612 is an original dataset announced via ProteomeXchange.

Title	Proteome and secretome analysis of human dorsal forebrain organoids reveals a time-dependent shift in the abundance of cell adhesion and extracellular matrix proteins
Description	Temporal dynamics of prenatal brain development are influenced by changes in the microenvironment. Particularly, extracellular proteins contribute to the dynamic niche that balances neural stem cell proliferation and differentiation. Here, we present a resource for proteome and secretome analysis of human induced pluripotent stem cell-derived dorsal forebrain organoids over the early developmental period. We used liquid chromatography-mass spectrometry to identify proteins found in whole organoid and secreted proteins at days 20, 35, and 50 of dorsal forebrain organoid differentiation. We show that the whole organoid proteome demonstrates progression in the neurodevelopmental trajectory with reduced proliferation and increased neural differentiation over time. However, secretome analysis revealed a unique signature for developmental progression. Cell adhesion molecules are enriched in the secretome of day 35 organoids, while secretome of day 50 organoids is enriched with extracellular matrix proteins, demonstrating a change in the extracellular interactions over time of organoid differentiation and maturation. We, therefore, show the relevance of secretome analysis for the thorough study of extracellular matrix-related proteins and the importance of time course study of neural organoids to understand the subtle changes that guide human neurodevelopment.
HostingRepository	MassIVE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_00:25:29.389.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohamed Ali Jarboui
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Acetyl; CarbamidomethylDTT; Met-loss; oxidation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-27 23:53:07	ID requested
⏵ 1	2024-11-05 00:25:29	announced

no publication

submitter keyword: Organoids, stem cells, neurodevelopment, neurogenesis, brain, neocortex, proteomics, secretome, extracellular proteins, cell adhesion, Proteomics, DIA, Secretome

Mohamed Ali Jarboui
contact affiliation	core facility medical proteomics, University klinikum Tuebingen
contact email	mohamed-ali.jarboui@uni-tuebingen.de
lab head
Mohamed Ali Jarboui
contact affiliation	University klinikum Tuebingen
contact email	mohamed-ali.jarboui@uni-tuebingen.de
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094878/