PXD052589-1

PXD052589 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of Babesia divergens extracellular vesicles
Description	Protozoan parasites of the genus Babesia are considered a serious threat to humans and animals worldwide. Babesia parasites are naturally transmitted by ticks and infect the erythrocytes or red blood cells (RBCs) of many vertebrates causing babesiosis. Infectious species include B. divergens, which causes asymptomatic to fatal babesiosis in humans and red water fever in cattle in Europe. To better understand the biology of B. divergens and to develop diagnostic and control strategies for babesiosis, we studied the extracellular vesicles (EVs) released into the environment by B. divergens-infected red blood cells (iRBCs). B. divergens parasites were cultured in human erythrocytes in vitro in a complete medium containing low human vesicle (LHV) serum. Uninfected erythrocytes (uRBC) were also maintained in the LHV complete medium. Bd-derived EVs and uRBC-derived EVs were collected and purified from the supernatants of B. divergens and uRBC cultures, respectively. Proteins were extracted from Bd-derived EVs and uRBC-derived EVs.
HostingRepository	PRIDE
AnnounceDate	2025-07-22
AnnouncementXML	Submission_2025-07-22_01:33:55.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Javier Sotillo
SpeciesList	scientific name: Babesia divergens; NCBI TaxID: 32595;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-27 05:17:23	ID requested
⏵ 1	2025-07-22 01:33:56	announced

Dataset with its publication pending

submitter keyword: proteomics, human red blood cells, protozoan, extracellulr vesicles,Babesia divergens

Luis Miguel González
contact affiliation	Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III
contact email	lmgonzal@isciii.es
lab head
Javier Sotillo
contact affiliation	Instituto de Salud Carlos III
contact email	javier.sotillo@isciii.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD052589

PRIDE project URI

• PRIDE
  1. PXD052589
     1. Label: PRIDE project
     2. Name: Proteomic characterization of Babesia divergens extracellular vesicles