PXD052551-1

PXD052551 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Differential alkylation of PHLPP2
Description	To determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-step differential alkylation experiment was compared to the 1-step alkylation reactions under fully denaturing conditions with iodoacetamide and methyl methanethiosulfonate.
HostingRepository	PRIDE
AnnounceDate	2025-03-13
AnnouncementXML	Submission_2025-03-13_02:26:01.984.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-24 02:54:47	ID requested
⏵ 1	2025-03-13 02:26:02	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: differential alkylation,Free thiols

submitter keyword: differential alkylation,Free thiols

Contact List

Dr. Thomas A. Leonard
contact affiliation	Department of Structural and Computational Biology, Max Perutz Labs, Campus Vienna Biocenter 5, 1030 Vienna, Austria
contact email	thomas.leonard@maxperutzlabs.ac.at
lab head
Markus Hartl
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria
contact email	proteomics@maxperutzlabs.ac.at
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD052551
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD052551

PRIDE project URI

Repository Record List

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