PXD052473-2

PXD052473 is an original dataset announced via ProteomeXchange.

Title	Molecular mechanisms of Acanthamoeba castellanii response to different sources of oxidative stress.
Description	Oxidative stress plays an important role in biology and is also an important factor in the pathogen-host relationship. The pathogenic free-living amoeba Acanthamoeba castellanii has several pathways to cope with reactive oxygen species and the damage they cause. In this study, we aimed to provide a comprehensive analysis of the amoeba's response to different sources of oxidative stress. Using whole-cell proteomic analysis, we obtained a complex picture of the changes in the proteome and identified potential key players in the defense against oxidative stress. Importantly, from the differential proteomics analysis we identified a candidate efflux pump that may be involved in Acanthamoeba drug resistance.
HostingRepository	PRIDE
AnnounceDate	2025-01-22
AnnouncementXML	Submission_2025-01-22_10:06:37.412.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Acanthamoeba castellanii; NCBI TaxID: 5755;
ModificationList	half cystine; monohydroxylated residue; deamidated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-22 03:25:52	ID requested
1	2025-01-21 08:35:53	announced
⏵ 2	2025-01-22 10:06:37	announced	2025-01-22: Updated project metadata.

10.1021/ACS.JPROTEOME.4C00573;

submitter keyword: drug resistance, ROS, oxidative stress,A. castellanii

Robert Sutak
contact affiliation	Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
contact email	robert.sutak@natur.cuni.cz
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052473
     1. Label: PRIDE project
     2. Name: Molecular mechanisms of Acanthamoeba castellanii response to different sources of oxidative stress.