PXD052450 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DSSBU: An MS-Cleavable Analogue of the BS3 Cross-Linker |
| Description | In this study, we introduce a new MS-cleavable cross-linker called disulfodisuccinimidyl dibutyric urea (DSSBU), which we developed in-house. DSSBU contains an N-hydroxysulfosuccinimide (sulfo-NHS) reactive group that primarily modifies lysine residues. It therefore serves as a water-soluble counterpart to the commercially available and widely used disuccinimidyl dibutyric urea (DSBU). To investigate the applicability of DSSBU, we compared the efficacy of four similar cross-linkers—bis[sulfosuccinimidyl] suberate (BS3), disuccinimidyl suberate (DSS), DSBU, and DSSBU—when used with the common model protein, bovine serum albumin (BSA). |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-29 |
| AnnouncementXML | Submission_2025-12-29_04:43:14.230.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Petra Junková |
| SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-05-21 15:18:51 | ID requested | |
| ⏵ 1 | 2025-12-29 04:43:14 | announced | |
Publication List
Keyword List
| submitter keyword: BSA, protein cross-linking, DSSBU cross-linker |
Contact List
| Josef Cvačka |
|---|
| contact affiliation | Josef Cvačka Group - Proteomics, Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Czech Republic |
| contact email | josef.cvacka@uochb.cas.cz |
| lab head | |
| Petra Junková |
|---|
| contact affiliation | Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague |
| contact email | petra.junkova@uochb.cas.cz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD052450 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052450
- Label: PRIDE project
- Name: DSSBU: An MS-Cleavable Analogue of the BS3 Cross-Linker
