PXD052438-1

PXD052438 is an original dataset announced via ProteomeXchange.

Title	Proteomic insights into human induced regulatory T cells: the role of LPXN and CD160 in immune suppression
Description	Regulatory T cells (Tregs) play a central role in maintaining immune homeostasis, and the implementation of in vitro induced Treg cells (iTreg) to control immune function has significant potential in clinical medicine. The clinical application of iTregs has been limited by their poor stability. To better define the molecular characteristics of human iTregs, we performed a data-independent acquisition proteomics, detecting over 8000 proteins and providing a quantitative comparison of their relative levels in iTregs and activated Th0 cells. Consistent with the known molecular characteristics of Tregs, several Treg signature proteins, including FOXP3, Eos, IL2RA, CTLA4, PD-1, Aiolos, LAG3, RUNX1 and HIC1, were identified and validated using targeted proteomics and/or qRT-PCR. Notably, leupaxin (LPXN) level was upregulated during Treg cell differentiation. Functional studies demonstrated that LPXN-deficient cells showed impaired expression of Treg protein markers FOXP3, Eos and IKZF3 and impaired suppression of effector T cells. In addition, we identified a distinct CD160⁺ iTregs subpopulation characterized by a distinct proteomic signature as compared to CD160⁻ iTregs. Together, these findings provide a high-resolution proteomic landscape of human iTregs and identified a novel role of LPXN in the development and suppressive activity of iTregs.
HostingRepository	PRIDE
AnnounceDate	2026-08-19
AnnouncementXML	Submission_2026-08-18_23:51:59.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kedar Batkulwar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-21 06:15:40	ID requested
⏵ 1	2026-08-18 23:51:59	announced
2	2026-08-20 23:32:25	announced	2026-08-21: Updated project metadata.

Dataset with its publication pending

submitter keyword: None

Riitta Lahesmaa
contact affiliation	Molecular Systems Immunology Group Turku Bioscience University of Turku P.O. Box 123, BioCity (Street adr. Tykistökatu 6 A, 7th floor) FIN-20521 Turku, Finland +358-40-718 4813 (Mobile)
contact email	rilahes@utu.fi
lab head
Kedar Batkulwar
contact affiliation	Turku Bioscience, University of Turku, Turku
contact email	kebatk@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD052438

PRIDE project URI

• PRIDE
  1. PXD052438
     1. Label: PRIDE project
     2. Name: Proteomic insights into human induced regulatory T cells: the role of LPXN and CD160 in immune suppression