PXD052421-1

PXD052421 is an original dataset announced via ProteomeXchange.

Title	Identification of downstream proteins of B3GNT5
Description	Pancreatic cancer (PC) is one of the most lethal solid malignancies and new therapeutic strategies are urgently needed. β1,3-N-acetylglucosaminyltransferase V (B3GNT5) may be a potential option for cancer treatment, but its role in pancreatic cancer remains unknown. We systematically verified the regulatory role of B3GNT5 on the malignant progression of pancreatic cancer and found that B3GNT5 overexpression significantly enhanced the malignant behaviors of tumor cells, such as invasion, migration, proliferation, stem cell properties, and drug resistance. In order to clarify the possible targets and molecular regulatory mechanisms of B3GNT5, we collected control and B3GNT5 overexpressing pancreatic cancer cells, analyzed the differential proteins by Label-free quantitative proteomics analysis. The Gene Ontology enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes were used to interpret the proteomic data. Finally, AKT1, MAPK1, and MAPK3 were identified as possible downstream targets of B3GNT5, which provides a more comprehensive perspective for understanding B3GNT5 aggravation of pancreatic cancer progression.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_17:02:38.042.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhe Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-20 19:57:55	ID requested
⏵ 1	2025-05-06 17:02:38	announced

10.1016/j.isci.2024.110889;

Yao W, Wang Y, Zhang X, Lin Y, B3GNT5 is a novel marker correlated with malignant phenotype and poor outcome in pancreatic cancer. iScience, 27(10):110889(2024) [pubmed]

submitter keyword: Human, LC-MS Label-free quantitative proteomics

Yuhe Lin
contact affiliation	Department of Oncology, Shengjing Hospital of China Medical University,Shenyang, People's Republic of China
contact email	linyuhe@sj-hospital.org
lab head
Yuhe Lin
contact affiliation	Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China
contact email	linyuhe@sj-hospital.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052421

PRIDE project URI

• PRIDE
  1. PXD052421
     1. Label: PRIDE project
     2. Name: Identification of downstream proteins of B3GNT5