PXD052417-1
PXD052417 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Heloderma horridum horridum venom |
Description | Heloderma horridum horridum commonly known as the scorpion lizard, Mexican scorpion, and beaded lizard, is a venomous reptile native of America. The venom derived from this lizard has potential applications, particularly in treatment of type II diabetes through the peptide Exendin. In this work, H. h. horridum venom was extracted from adult specimens and lyophilized. To characterize the venom, enzymatic assays, including hyaluronidase, phospholipase A2, and proteolytic activity were conducted. A proteomic analysis of the venom was also performed employing bottom-up/shotgun approaches from SDS-PAGE and High pH Reversed-Phase chromatography., besides fractionation of tryptic peptides using a nano-LC-MS/MS. These approaches involved massive sequencing to enhancing the likelihood of detecting an extensive range of venom proteins. The proteins and peptides found in H. h. horridum venom are reviewed according to the classification of the transcriptome previously reported. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-11 |
AnnouncementXML | Submission_2024-08-10_16:07:47.729.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD052417 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Miguel Elizalde |
SpeciesList | scientific name: Heloderma horridum horridum; NCBI TaxID: 8552; |
ModificationList | monohydroxylated residue; deaminated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-05-20 16:27:07 | ID requested | |
⏵ 1 | 2024-08-10 16:07:48 | announced |
Publication List
10.1021/acs.jproteome.4c00287; |
Lino-L, ó, pez GJ, Ruiz-May E, Elizalde-Contreras JM, Jim, é, nez-Vargas JM, Rodr, í, guez-V, á, zquez A, Gonz, á, lez-Carrillo G, Boj, ó, rquez-Vel, á, zquez E, Garc, í, a-Villalvazo PE, Berm, ú, dez-Guzm, á, n MJ, Zatarain-Palacios R, V, á, zquez-Vuelvas OF, Valdez-Vel, á, zquez LL, Corzo G, Venom: Assessment to Its Transcriptome and Newfound Proteins. J Proteome Res, 23(8):3638-3648(2024) [pubmed] |
10.6019/PXD052417; |
Keyword List
submitter keyword: Lizard, Proteome, Enzymatic activities, Venom |
Contact List
Eliel Ruiz-May | |
---|---|
contact affiliation | Instituto de Ecología, Carretera antigua a Coatepec 351, El Haya, Xalapa, Veracruz, México. |
contact email | eliel.ruiz@inecol.mx |
lab head | |
Miguel Elizalde | |
contact affiliation | Instituto de Ecolog'ia A.C. |
contact email | jose.elizalde@inecol.mx |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD052417 |
PRIDE project URI |
Repository Record List
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