PXD052400 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SMAX1/SMXL2 interacting proteins in Arabidopsis seedling through IP-MS |
Description | Karrikins (KARs) are a class of butenolide molecules found in smoke. KARs regulate many aspects of plant development and environmental responses, including Seed germination, hypocotyl elongation, leaf shape, root development, and resistance to drought, salt, and heat stress. The transcriptional repressor proteins SUPPRESSOR OF MAX2 1 (SMAX1) and SMAX1-like2 (SMXL2) integrate karrikin signaling by repressing gene expression through direct binding to promoters or through inhibiting activities of transcription factors. To elucidate the mechanism by which SMAX1 and SMXL2 mediate KAR signaling, we performed immunoprecipitation followed by mass spectrometry (IP-MS) analysis and identified peptides in transgenic seedlings that overexpress GFP, SMAX1-GFP, and SMXL2-GFP, respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:46:18.345.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | wenwen chang |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-20 02:02:16 | ID requested | |
1 | 2024-06-22 03:28:02 | announced | |
⏵ 2 | 2024-10-22 06:46:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
Chang W, Qiao Q, Li Q, Li X, Li Y, Huang X, Wang Y, Li J, Wang B, Wang L, Non-transcriptional regulatory activity of SMAX1 and SMXL2 mediates karrikin-regulated seedling response to red light in Arabidopsis. Mol Plant, 17(7):1054-1072(2024) [pubmed] |
10.1016/j.molp.2024.05.007; |
Keyword List
submitter keyword: SMAX1, Arabidopsis thaliana,IP-MS, SMXL2, Seedling, Karrikin |
Contact List
Lei Wang |
contact affiliation | Institute of Genetics and Developmental Biology Center for Agricultural Resources Research, Shijiazhuang, Hebei CHINA |
contact email | wanglei@sjziam.ac.cn |
lab head | |
wenwen chang |
contact affiliation | Institute of Genetics and Developmental Biology Center for Agricultural Resources Research |
contact email | changwenwen18@mails.ucas.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052400
- Label: PRIDE project
- Name: SMAX1/SMXL2 interacting proteins in Arabidopsis seedling through IP-MS