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PXD052393-1

PXD052393 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass spectrometry analysis of potential interactors or RNF214
Description	We established an APEX proximity labeling strategy in coupled with mass spectrometry to identify interacting proteins of RNF214. In this approach, we first fused an engineered ascorbate peroxidase (APEX2) to either N-terminus or C-terminus of RNF214, expressed these two fusion proteins in HLF, an HCC cell line, near the endogenous level, and generated short-lived radicals around the APEX2-RNF214 fusion proteins to label biotin on nearby interactive proteins by adding hydrogen peroxide (H2O2) and biotin-phenol (also called biotin-tyramide) transiently. Biotinylated proteins were then isolated using Streptavidin resin for protein identification by mass spectrometry.
HostingRepository	iProX
AnnounceDate	2024-05-18
AnnouncementXML	Submission_2024-05-20_00:01:14.918.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengjia Lin
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-20 00:00:46	ID requested
⏵ 1	2024-05-20 00:01:15	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: APEX2

Contact List

Jianping Jin
contact affiliation	Zhejiang University
contact email	jianping_jin@zju.edu.cn
lab head
Mengjia Lin
contact affiliation	Zhejiang University
contact email	linmengjia9@zju.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI