PXD052390-1
PXD052390 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Genome-scale CRISPRi screen identifies pcnB repression conferring overproduction of free fatty acids in Escherichia coli |
| Description | In this study, we carried out genome-scale CRISPRi modulation combining fluorescence-activated cell sorting (FACS) and next-generation sequencing (NGS) to identify genetic determinants for FFAs overproduction in Escherichia coli. The pcnBi (AP) strain that repressed the expression of pcnB produced 2992 mg l FFAs, which was 4.0-fold of the CF (A) strain. To analyze the underlying mechanism of FFAs overproduction, the engineered strain pcnBi and the control strain CF were applied to the transcriptomic and proteomic analyses. |
| HostingRepository | iProX |
| AnnounceDate | 2024-05-18 |
| AnnouncementXML | Submission_2024-05-19_22:38:17.919.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lixia Fang |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-05-19 22:37:55 | ID requested | |
| ⏵ 1 | 2024-05-19 22:38:18 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: CRISPRi libarary, target identification, free fatty acids, pcnB, Escherichia coli |
Contact List
| Yingxiu Cao | |
|---|---|
| contact affiliation | Tianjin University |
| contact email | caoyingxiu@tju.edu.cn |
| lab head | |
| Lixia Fang | |
| contact affiliation | Tianjin University |
| contact email | lxfang@tju.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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