PXD052356-1

PXD052356 is an original dataset announced via ProteomeXchange.

Title	Brain tissue Protein Profile of Periplaneta americana exposed to commercial pesticide
Description	Various organic and natural pesticides play a vital role in insect control, leading to variation in their protein profile (Abubakar et al., 2019; James & Xu, 2012). The sex, size, weight, and growth conditions could be the possible factors associated with fluctuations in the concentration of protein production in the cockroach (Bouchebtiet al., 2022) The current study of Perplanata americana under pesticide activity considered to observe the proteomics of Brain and protein expression under stressed conditions. 15 cockroaches were exposed to 5g of Laxman Rekha (11:1 ratio) to assess the impact of pesticide on protein synthesis. National and international research conducted on aqueous and alcoholic extraction of gut, exoskeleton and brain protein of Periplaneta spp. and Blatta spp have revealed 10 potential antibacterial proteins between 18 kDa to 72kDa range. These proteins were purified using chromatographic techniques like single dimension reducing PAGE, FPLC-MS, LC-MS, MALDI TOF-MS etc. The current study is aimed at isolation, identification and characterisation of proteins from cockroach brain tissue lysate using Liquid chromatography and Orbitrap Mass spectrometer technique. The data analysis was done using Thermo Proteome Discoverer software version 2.2. 0.388. The database used was Uniprot-Periplaneta americana.
HostingRepository	PRIDE
AnnounceDate	2024-06-27
AnnouncementXML	Submission_2024-06-27_01:57:12.351.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052356
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Siddharth Sagar
SpeciesList	scientific name: Periplaneta americana; NCBI TaxID: 6978;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-17 11:04:47	ID requested
⏵ 1	2024-06-27 01:57:12	announced
2	2024-10-22 06:47:38	announced	2024-10-22: Updated project metadata.

10.6019/PXD052356;

submitter keyword: Pesticide, Brain Tissue, LC-MS/MS

Dr.Jayaprada Rao
contact affiliation	Department of Biotechnology,Mithibai College,India
contact email	jayaprada_chunduri@hotmail.com
lab head
Siddharth Sagar
contact affiliation	Co-founder and Professor at Prarambh Science Academy, Sangli
contact email	siddharth.sgr@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052356
     1. Label: PRIDE project
     2. Name: Brain tissue Protein Profile of Periplaneta americana exposed to commercial pesticide