PXD052350-1

PXD052350 is an original dataset announced via ProteomeXchange.

Title	Linking planar polarity signaling directly to actomyosin contractility during vertebrate neurulation
Description	Actomyosin contractility represents an ancient feature of eukaryotic cells participating in many developmental and homeostasis events, including tissue morphogenesis, muscle contraction, and cell migration, with dysregulation implicated in various pathological conditions, such as cancer. At the molecular level, actomyosin comprises actin bundles and myosin motor proteins that are sensitive to posttranslational modifications like phosphorylation. While the molecular components of actomyosin and its regulation are well understood, the coordination of contractility by extracellular and intracellular signals, particularly from cellular signaling pathways, remains incompletely elucidated. This study focuses on planar cell polarity (PCP) signaling, previously associated with actomyosin contractility during vertebrate neurulation. Contrary to previous studies, our investigation reveals that main cytoplasmic PCP proteins, Prickle and Dishevelled, interact directly with key actomyosin components such as myosin light chain (MLC), leading to its phosphorylation and localized activation. Through relevant in vitro and in vivo models, we demonstrate that these PCP proteins function not merely passively and indirectly as previously reported, but that they actively control actomyosin contractility by acting as scaffold proteins, orchestrating the necessary machinery for contractility. These findings unveil a direct link of PCP signaling in crucial actomyosin contractility processes through MLC that are relevant to vertebrate neurulation and potentially beyond.
HostingRepository	PRIDE
AnnounceDate	2024-09-19
AnnouncementXML	Submission_2024-09-19_03:47:06.130.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Roudnický
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-17 07:10:09	ID requested
⏵ 1	2024-09-19 03:47:06	announced
2	2024-10-22 06:59:03	announced	2024-10-22: Updated project metadata.

10.1098/RSOB.20160251;

submitter keyword: planar cell polarity, Dishevelled, Exploris 480, vertebrates, neurulation, Proteomics, Prickle,actomyosin contractility

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Pavel Roudnický
contact affiliation	Masaryk University
contact email	p.roudnicky@mail.muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052350
     1. Label: PRIDE project
     2. Name: Linking planar polarity signaling directly to actomyosin contractility during vertebrate neurulation