PXD052319-1

PXD052319 is an original dataset announced via ProteomeXchange.

Title	MicroRNAs Biomarkers and Pathway Enrichment Analysis in Severe Pulmonary Hemorrhagic Syndrome due to Leptospirosis: A Multi-Omics and Multi-Center Study
Description	Background. Leptospirosis is among the most widespread zoonoses worldwide. Severe pulmonary hemorrhagic syndrome (SPHS) represents a serious complication of leptospirosis, with poor understanding of its underlying mechanisms and an urgent need for identification of effective biomarkers. Methods. A nested case-control analysis of the blood specimens obtained from two previous multi-center cohorts was conducted. Candidate microRNAs were initially discovered through a global profiling of 800 serum microRNAs, then validated using real-time polymerase-chain reactions. We further conducted a multi-omics analysis incorporating transcriptomic and proteomic data to identify enriched pathways through which the newly identified microRNAs could regulate. Findings. A total of 28 SPHS and 140 non-SPHS patients were evaluated by serum microRNA profiling, revealing distinct expression patterns between the two phenotypes. From the top 81 significantly expressed microRNAs, seven were selected for validation. Among these, miR-5010-3p and miR-147b-3p had area under the curve (AUC) values of 0.72 (95% CI: 0·62–0·81) and 0·66 (95% CI: 0·55–0·76) for discriminating SPHS. Notably, the two microRNAs could detect SPHS in patients who were yet to manifest chest radiograph shadows at the time of sample collection, and in a subgroup of patients who were recruited on day 2 of illness or earlier, with consistent AUC values. Integrated gene-protein pathway enrichment analysis revealed numerous pathways involving host immune responses. Among these, the tumor necrosis factor signaling pathway was the most significant with many of its member genes being targeted by miR-5010-3p or miR-147b-3p.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:08:30.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PHU TRAN
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Leptospira; NCBI TaxID: NCBITaxon:171;
ModificationList	No PTMs are included in the dataset
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 08:14:59	ID requested
⏵ 1	2026-02-08 16:08:31	announced

10.1016/j.jinf.2024.106400;

Tran PNT, Limothai U, Dinhuzen J, Tachaboon S, Sukmark T, Dokpong C, Roytrakul S, Haake DA, Srisawat N, MicroRNA biomarkers and host response pathways in severe pulmonary hemorrhagic syndrome due to leptospirosis: A multi-omics study. J Infect, 90(2):106400(2025) [pubmed]

submitter keyword: Leptospirosis, multi-omics, pulmonary hemorrhage

Phu Nguyen Trong Tran
contact affiliation	Faculty of medicine, Chulalongkorn University
contact email	tntphu@ctump.edu.vn
lab head
PHU TRAN
contact affiliation	Chulalongkorn University
contact email	tntphu@ctump.edu.vn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052319
     1. Label: PRIDE project
     2. Name: MicroRNAs Biomarkers and Pathway Enrichment Analysis in Severe Pulmonary Hemorrhagic Syndrome due to Leptospirosis: A Multi-Omics and Multi-Center Study