PXD052307-1

PXD052307 is an original dataset announced via ProteomeXchange.

Title	Ribosome-inactivation by a widely distributed class of C-tail anchored membrane proteins
Description	Ribosome hibernation is a commonly used strategy that protects ribosomes under unfavorable conditions and regulates developmental processes. Multiple ribosome-hibernation factors have been identified in all domains of life, but due to their structural diversity and the lack of a common mechanism by which they inactivate ribosomes, it is currently unknown how many different hibernation factors exist. Here, we demonstrate that the YqjD/ElaB/YgaM protein family constitute an abundant class of ribosome-hibernating proteins and represent the first membrane-bound hibernating factors identified in bacteria. Our data demonstrate that they interact with 50S ribosomal subunit and show a concentration-dependent inhibition of protein synthesis. By combining in vivo cross-linking with mass spectrometry, we show that they bind via their N-termini to proteins that surround the ribosomal tunnel exit and even penetrate into the ribosomal tunnel. Thus, YqjD/ElaB/YgaM inhibit translation by blocking the ribosomal tunnel and thus mimic the activity of antimicrobial peptides and macrolide antibiotics.
HostingRepository	PRIDE
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-03-04_05:50:30.948.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052307
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Friedel Drepper
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 05:34:09	ID requested
⏵ 1	2025-03-04 05:50:31	announced

10.1016/J.STR.2024.09.019;

10.6019/PXD052307;

submitter keyword: translation

stress response, YgaM, macrolide antibiotics,YqjD, ElaB, antimicrobial peptides

Friedel Drepper
contact affiliation	Faculty of Biology, University of Freiburg, Freiburg, Germany
contact email	f.drepper@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliation	University of Freiburg
contact email	f.drepper@biologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052307
     1. Label: PRIDE project
     2. Name: Ribosome-inactivation by a widely distributed class of C-tail anchored membrane proteins