PXD052250-1

PXD052250 is an original dataset announced via ProteomeXchange.

Title	Persistent epithelial barrier dysfunction and Periostin accumulation in histologic remission Eosinophilic Esophagitis
Description	Patients with Eosinophilic esophagitis (EoE) require long-lasting resolution of inflammation to prevent fibrostenosis and dysphagia. However, the dissociation between symptoms and histologic improvement suggests persistent molecular drivers despite remission. We aimed to characterize persisting molecular alterations in pediatric patients with EoE using tissue proteomics. Esophageal tissue biopsies (n=151) and clinical data were collected prospectively from pediatric patients with EoE (N=34), gastroesophageal reflux disease (GERD; N=10; inflammatory controls) or functional disorders (FD; N=20; non-inflammatory controls). Biospies (n=131) acquired from the diagnostic endoscopy and up to 7 follow-ups were considered for proteome analysis in patients with EoE, while for GERD and FD only biopsies from initial diagnosis were included. Histologically active EoE (15 eosinophils per high power field (hpf)) was diagnosed in 79 biopsies and 52 samples derived from patients with EoE in remission (15 eosinophils per hpf). After microdissection of the epithelium proteins were extracted from the esophageal tissue followed by a liquid chromatography-tandem mass spectrometry. Proteomic analysis identified 3,704 different proteins in total across all samples.
HostingRepository	PRIDE
AnnounceDate	2024-09-23
AnnouncementXML	Submission_2024-09-23_03:30:44.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-14 03:00:27	ID requested
⏵ 1	2024-09-23 03:30:44	announced

Dataset with its publication pending

submitter keyword: Fibrosis, molecular remission, histologic remission, Proteome, Epithelial barrier dysfunction,Eosinophilic Esophagitis

Tobias Schwerd, MD
contact affiliation	Department of Pediatrics Dr. von Hauner Children’s Hospital University Hospital LMU Munich Lindwurmstr. 4 80337 Munich, Germany
contact email	tschwerd@med.lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD052250

PRIDE project URI

• PRIDE
  1. PXD052250
     1. Label: PRIDE project
     2. Name: Persistent epithelial barrier dysfunction and Periostin accumulation in histologic remission Eosinophilic Esophagitis