PXD052233-1

PXD052233 is an original dataset announced via ProteomeXchange.

Title	Hepatovirus translation requires PDAP1, an eIF4E-binding protein regulating ER stress responses
Description	The overexpression and misfolding of viral proteins in the endoplasmic reticulum (ER) may cause cellular stress, thereby inducing cytoprotective proteostatic host responses involving phosphorylation of eIFa. Here, we show the positive-strand RNA virus responsible for infectious hepatitis adopts a stress-resistant, phospho-eIF2a independent mechanism of translation that ensures synthesis of its viral proteins within the infected liver. Cap-independent hepatovirus translation and productive infection in vivo requires PDAP1, a small phosphoprotein with previously unrecognized eIF4E-binding activity. We show PDAP1 interacts also with eIF1A, and is essential for translation of stress-resistant host mRNAs that evade the proteostatic response to ER stress.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_16:33:48.966.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Xie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-13 12:54:50	ID requested
⏵ 1	2025-05-06 16:33:49	announced

Shirasaki T, Lenarcic E, Misumi I, Xie L, Fusco WG, Yonish B, Das A, Kim H, Cameron CE, L, é, ger-Abraham M, Chen X, Cullen JM, Whitmire JK, Li Y, Duncan JA, Moorman NJ, Lemon SM, Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses. Sci Adv, 10(47):eadq6342(2024) [pubmed]

10.1126/sciadv.adq6342;

submitter keyword: hepatovirus, LC-MS,PDAP1, eIFa

Stanley M. Lemon
contact affiliation	Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
contact email	smlemon@med.unc.edu
lab head
Ling Xie
contact affiliation	UNC at Chapel Hill
contact email	xiel@email.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052233

PRIDE project URI

• PRIDE
  1. PXD052233
     1. Label: PRIDE project
     2. Name: Hepatovirus translation requires PDAP1, an eIF4E-binding protein regulating ER stress responses