PXD052221-2

PXD052221 is an original dataset announced via ProteomeXchange.

Title	Membrane prewetting by condensates promotes tight junction belt formation
Description	Time-resolve proximity proteomics of tight junction. To understand how the tight junction belt is assembled and positioned, we combined APEX2 proximity proteomics of the main junctional scaffold protein ZO-1 with a calcium switch tissue formation assay.This combination allowed us to synchronize the initiation of junction assembly in the entire tissue by the addition of calcium to the culture medium and quantify the time evolution of the junctional proteome during the assembly process using proximity proteomics.
HostingRepository	PRIDE
AnnounceDate	2024-09-10
AnnouncementXML	Submission_2024-09-10_06:49:00.580.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karina pombo garcia
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-13 07:48:09	ID requested
1	2024-05-22 02:23:30	announced
⏵ 2	2024-09-10 06:49:01	announced	2024-09-10: Updated project metadata.

10.1101/2022.12.16.520750;

10.1038/s41586-024-07726-0;

Pombo-Garc, í, a K, Adame-Arana O, Martin-Lemaitre C, J, ü, licher F, Honigmann A, Membrane prewetting by condensates promotes tight-junction belt formation. Nature, 632(8025):647-655(2024) [pubmed]

submitter keyword: Canis Lupus, tight junction, TMT, APEX2, MDCK-II

karina pombo garcia
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetics
contact email	Karina.pombo-garcia@mpi-cbg.de
lab head
Karina pombo garcia
contact affiliation	MPI-CBG Max Planck Institute of molecular cell biology and genetics
contact email	karina.pombo-garcia@mpi-cbg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD052221

PRIDE project URI

• PRIDE
  1. PXD052221
     1. Label: PRIDE project
     2. Name: Membrane prewetting by condensates promotes tight junction belt formation