PXD052198 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MFF budding from mitochondria regulates melanosome size and maturation |
| Description | Melanosomes are lysosome-related organelles that produce and accumulate melanin. Their biogenesis begins with the trafficking of melanogenic proteins from the endo-lysosomal system into non-pigmented vacuoles derived from endosomes. Subsequently, melanosome maturation is regulated by their association with mitochondria and requires the export and recycling of unneeded cargo via tubular carriers and fission, the mechanisms of which are unknown. Here we show that the outer mitochondrial membrane protein mitochondrial fission factor (MFF) is actively released from mitochondria to melanosomes. We found MFF on melanosomes at different stages of maturation and early melanosome fission sites. Upon downregulation of MFF, but not DRP1, early melanosomes enlarged, intracellular melanin accumulated and melanosome lumen catabolism increased. The MFF interactome in melanocytes posited a role for a complex network of cytoskeletal factors in its activity. Inhibition of actin nucleation was sufficient to restore the effects of MFF silencing on melanosomes. Our data unveil an extramitochondrial role for MFF in the regulation of melanosome morphology and maturation that, independently of DRP1, is supported by actin polymerization and nucleation factors. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-20 |
| AnnouncementXML | Submission_2026-03-20_10:58:24.546.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Marta Giacomello |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-05-11 10:31:37 | ID requested | |
| ⏵ 1 | 2026-03-20 10:58:25 | announced | |
Publication List
Keyword List
Contact List
| Marta Giacomello |
|---|
| contact affiliation | University of Padova |
| contact email | marta.giacomello@unipd.it |
| lab head | |
| Marta Giacomello |
|---|
| contact affiliation | University of Padova, Dept of Biology |
| contact email | marta.giacomello@unipd.it |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD052198 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052198
- Label: PRIDE project
- Name: MFF budding from mitochondria regulates melanosome size and maturation
