PXD052172-1

PXD052172 is an original dataset announced via ProteomeXchange.

Title	A chemoenzymatic method for simultaneous profiling N- and O-glycans on glycoproteins using one-pot format
Description	Glycosylation, including N-glycosylation and O-glycosylation is generally characterized and controlled as a critical quality attribute for therapeutic glycoproteins because glycans can impact protein-based drug product efficacy, half-life, stability, and safety. Analytical procedures to characterize N-glycans are relatively well-established, but the characterization of O-glycans is challenging due to the complex workflows and lack of enzymatic tools. Here we present a simplified chemoenzymatic method to simultaneously profile N- and O-glycans from the same sample using a one-pot format by mass spectrometry (MS). N-glycans were first released by PNGase F, followed by O-glycopeptide generation by Proteinase K, selective N-glycan reduction, and O-glycan release by β-elimination during permethylation of both N- and O- glycans. Glycan structural assignments, and determination of N- to O-glycan ratio was obtained from the one-pot mass spectra. The streamlined, one-pot method is an accurate and reproducible approach that will facilitate advanced characterizations for quality assessments of therapeutic glycoproteins.
HostingRepository	PRIDE
AnnounceDate	2024-08-01
AnnouncementXML	Submission_2024-08-01_11:41:50.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Bettinger
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; deaminated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-10 06:39:15	ID requested
⏵ 1	2024-08-01 11:41:50	announced

Dataset with its publication pending

submitter keyword: glycomics, glycoproteomics, fetuin

Tongzhong Ju
contact affiliation	FDA
contact email	tongzhong.ju@fda.hhs.gov
lab head
John Bettinger
contact affiliation	FDA
contact email	john.bettinger@fda.hhs.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052172
     1. Label: PRIDE project
     2. Name: A chemoenzymatic method for simultaneous profiling N- and O-glycans on glycoproteins using one-pot format