PXD052154-2

PXD052154 is an original dataset announced via ProteomeXchange.

Title	The temporal dynamics of proteins in aged skin wound healing and comparison to gene expression
Description	Wound healing, a multifaceted process, often leads to chronic wounds when disrupted. Predominantly, research in this area has relied on in vitro or animal models, which potentially oversimplify the intricacies of human wound healing. This simplification could contribute to the current gap in effective clinical therapies and prognostic tools. Our study aims to deepen the understanding of human skin wound healing mechanisms at the temporal protein level. Utilizing labeled mass spectrometry, we quantified the proteome of full-thickness skin samples at both acute and late healing stages from legs of 14 healthy individuals. Our results revealed distinct proteomic profiles corresponding to different wound healing days, indicating a unique protein signature for each stage. Functional enrichment analysis identified key elements such as post-translational modifications, histone interaction networks, and proteins related to transcription and metabolism as pivotal in the early phases of healing. Additionally, a comparative analysis of protein and mRNA expression in similar sample types revealed only moderate correlation, suggesting that mRNA assessments alone might not fully capture the complexity of the healing process. This finding underscores the necessity for multi-omics approaches to comprehensively understand complex molecular mechanisms such as human skin wound healing.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:29.766.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Monica Torres
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-09 14:42:46	ID requested
1	2024-10-02 06:21:27	announced
⏵ 2	2024-10-22 07:00:30	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, TMT, MS,human skin wound healing

Jakob Wikström
contact affiliation	Department of Medicine Solna, Karolinska Institutet. Dermatovenereology clinic, Karolinska University Hospital.
contact email	jakob.wikstrom@ki.se
lab head
Monica Torres
contact affiliation	Karolinska Institute
contact email	monica.barbara.torres@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD052154

PRIDE project URI

• PRIDE
  1. PXD052154
     1. Label: PRIDE project
     2. Name: The temporal dynamics of proteins in aged skin wound healing and comparison to gene expression