PXD052115-1

PXD052115 is an original dataset announced via ProteomeXchange.

Title	hnRNP R promotes O-GlcNAcylation of eIF4G to facilitate axonal protein synthesis
Description	Motoneurons critically depend on precise spatial and temporal control of translation for axon growth and the establishment and maintenance of neuromuscular connections. While defects in local translation have been implicated in the pathogenesis of motoneuron disorders, little is known about mechanisms regulating axonal protein synthesis. Here, we report that motoneurons derived from Hnrnpr knockout mice show reduced axon growth accompanied by lowered synthesis of cytoskeletal and synaptic components in axons. Mutant mice display denervated neuromuscular junctions and impaired motor behavior. In axons, hnRNP R is a component of translation initiation complexes and, through interaction with O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (Ogt), modulates O-GlcNAcylation of eIF4G, thereby stimulating translation. Restoring axonal O-GlcNAc levels rescued local protein synthesis and axon growth defects of hnRNP R knockout motoneurons. Together, these findings demonstrate a function of hnRNP R in controlling the local production of key factors required for axon growth and formation of neuromuscular innervations.
HostingRepository	PRIDE
AnnounceDate	2024-08-22
AnnouncementXML	Submission_2024-08-22_07:34:05.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-08 08:12:37	ID requested
⏵ 1	2024-08-22 07:34:05	announced

Dataset with its publication pending

submitter keyword: RL2, axonal translation,OGT, interactome, motoneuron

Matthias Mann
contact affiliation	Max Planck Institute of Biochemistry Am Klopferspitz 18, 82152 Planegg
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052115
     1. Label: PRIDE project
     2. Name: hnRNP R promotes O-GlcNAcylation of eIF4G to facilitate axonal protein synthesis