PXD052101-1

PXD052101 is an original dataset announced via ProteomeXchange.

Title	A “one-step probe” enables the global and simultaneous mapping of N-glycosylation, core fucosylation and truncated O-glycosylation
Description	Protein glycosylation, mainly divided into N-glycosylation and O-glycosylation, is the most prevalent and complex protein posttranslational modification. Mapping of glycosylation sites where glycans attached to proteins is the prerequisite to un-cover the biological functions that glycans played in living cells. However, profiling of multiple glycosylations from a given biological sample is still a challenging issue in glycoscience. Here, a strategy integrates one-step enzymatic labeling and multi-steps release was developed to enable simultaneous profiling of N-glycosylation and truncated O-glycosylation by mass spec-trometry (MS) technology. A probe functionalized with temperature-sensitive polymer and a cleavable linker can be tagged to N-glyans and O-glycans by two substrate-specific sialyltransferases in a one-step manner. Without the need of additional biorthogonal reaction to introduce enrichment tag or resin, the labelled peptides can be directly precipitated out of the solu-tion in water bath to achieve enrichment (0 °C). Then, the captured glycpetides were released by endoglycosidase and UV356 irradiation sequentially and analyzed by MS. After searching database using different variable modifications, glyco-proteomes including N-glycosylation, core fucosylation and truncated mucin-type O-glycosylation can be profiled successfully.
HostingRepository	iProX
AnnounceDate	2024-05-07
AnnouncementXML	Submission_2024-05-08_18:51:52.228.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuqiu Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-07 19:48:21	ID requested
⏵ 1	2024-05-08 18:51:52	announced

Dataset with its publication pending

submitter keyword: N-glycosylation, core fucosylation, O-glycosylation, one-step probe

Hu Zhou
contact affiliation	Shanghai Institute of Materia Medica
contact email	zhouhu@simm.ac.cn
lab head
Yuqiu Wang
contact affiliation	Shanghai Institute of Materia Medica
contact email	995752924@qq.com
dataset submitter

iProX dataset URI