PXD052100-1

PXD052100 is an original dataset announced via ProteomeXchange.

Title	Amiloride reduces FN3K expression to restore sunitinib sensitivity in renal cell carcinoma
Description	The kidney is a vital organ responsible for water and sodium metabolism, while the primary function of amiloride is to promote the excretion of water and sodium. We investigated amiloride enhanced the sunitinib sensitivity in RCC. We found both sunitinib and amiloride displayed cytotoxicity and exerted the synergy effect in RCC cells in vivo and in vitro arrays. Protein expression profiles were screened via MS/TMT, revealing that FN3K was upregulated in sunitinib group, and rescued in amiloride and the combination administration. Exogenous FN3K could promote proliferation, invasion and metastasis and decrease the sensitivity of Caki-1 cells to sunitinib, also, exogenous FN3K up-regulated VEGFR2 expression and activated AKT/mTOR signal pathway. More FN3K and VEGFR2 accumulated in R-Caki-1 cells and rescued by amiloride treatment. Co-IP and IF confirmed the interaction between FN3K and VEGFR2. In conclusion, FN3K depletion mediated VEGFR2 disruption promotes amiloride synergized the anti-RCC activity of sunitinib.
HostingRepository	iProX
AnnounceDate	2024-05-07
AnnouncementXML	Submission_2024-12-09_18:59:00.365.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongyang Wu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-07 19:44:46	ID requested
⏵ 1	2024-12-09 18:59:01	announced

Bai Y, You Y, Chen D, Chen Y, Yin Z, Liao S, You B, Lu D, Sun Y, Wu L, Wu Y, Amiloride reduces fructosamine-3-kinase expression to restore sunitinib sensitivity in renal cell carcinoma. iScience, 27(6):109997(2024) [pubmed]

submitter keyword: Renal cell carcinoma, Sunitinib resistance, Amiloride, FN3K, VEGFR2

Yongyang Wu
contact affiliation	Affiliated Sanming First Hospital, Fujian Medical University
contact email	wuyyfj@fjmu.edu.cn
lab head
Yongyang Wu
contact affiliation	Affiliated Sanming First Hospital, Fujian Medical University
contact email	wuyyfj@fjmu.edu.cn
dataset submitter

iProX dataset URI