PXD052083-1

PXD052083 is an original dataset announced via ProteomeXchange.

Title	GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway
Description	Glycosylation plays an important role for modifications of lipids and sorting of proteins that is regulated by asymmetric intra-Golgi distribution and SPPL3-mediated cleavage of Golgi enzymes. We found that cells lacking the Golgi N-acetylglucosamine phosphotransferase (GNPT) retention factor LYSET/TMEM251 display a SPPL3-dependent hypersecretion of the galactosyltransferase B4GALT5 which is caused by the loss of its lysosomal mannose 6-phosphate (M6P) targeting signal. Similarly, loss of the COPI adaptors GOLPH3/GOLPH3L destabilized LYSET-GNPT complexes and led to a prominent hypersecretion of B4GALT5 and lysosomal enzymes. Mechanistically, we identified LYSET as a novel, atypical client of GOLPH3/GOLPH3L. GOLPH3/GOLPH3L hence ensure cis-Golgi localization of the LYSET-GNPT complex and maintain Golgi polarity, integrity of the M6P tagging machinery and homeostasis of lysosomes.
HostingRepository	PRIDE
AnnounceDate	2024-11-27
AnnouncementXML	Submission_2024-11-27_03:07:54.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	jiaran Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-07 10:20:32	ID requested
⏵ 1	2024-11-27 03:07:55	announced

Brauer BK, Chen Z, Beirow F, Li J, Meisinger D, Capriotti E, Schweizer M, Wagner L, Wienberg J, Hobohm L, Blume L, Qiao W, Narimatsu Y, Carette JE, Clausen H, Winter D, Braulke T, Jabs S, Voss M, GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway. EMBO J, 43(24):6264-6290(2024) [pubmed]

10.1038/s44318-024-00305-z;

submitter keyword: Golgi apparatus

lysosomes

mannose 6-phophate tagging

glycosyltransferase secretion

intramembrane proteolysis

COPI vesicles

Dominic Winter
contact affiliation	Institute of biochemistry and molecular biology, Bonn
contact email	dwin@uni-bonn.de
lab head
jiaran Li
contact affiliation	Institute of biochemistry and molecular biology
contact email	jiaranli@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052083
     1. Label: PRIDE project
     2. Name: GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway