PXD052072-1

PXD052072 is an original dataset announced via ProteomeXchange.

Title	Aged Mouse Muscle: Sedentary or Exercised Animals with or without Nicotinamide N-methyltransferase Inhibitor Treatment
Description	Aged (22-mo) female mice (n=35), obtained from the US NIH National Institute on Aging (NIA) Aged Rodent Colony, were randomly assigned to one of four groups: saline-treated sedentary ( n=9), nicotinamide N-methyltransferase inhibitor (NNMTi)-treated sedentary (10 mg/kg body weight; n=9), saline-treated progressive weighted wheel running (PoWeR; saline; n=10), and NNMTi-treated PoWeR (10 mg/kg body weight; n=7) . Group-housed Sed cohorts were compared to singly-housed PoWeR cohorts that underwent a 1-week introduction to an unweighted wheel, followed by eight weeks of weighted wheel running. Forelimb grip strength was assessed by a single NNMTi treatment-blinded investigator during week six and averaged across 2-4 trials/mouse. At the end of week 8, when mice were ~24.5-months-old, the strength of the right limb plantarflexor muscle complex was measured using an in vivo isometric peak tetanic torque technique and a fatigue test. After the fatigue test, mice were euthanized, and tissues were weighed and collected. Of note, hindlimb muscles from the right limb (the limb that underwent in vivo isometric peak tetanic torque and fatigue testing) were processed for immunohistochemistry to avoid acute effects of muscle functional testing on the proteome and metabolome. Hindlimb muscles from the left limb that did not undergo torque and fatigue testing were flash-frozen for proteome and metabolome analyses.
HostingRepository	MassIVE
AnnounceDate	2024-07-23
AnnouncementXML	Submission_2024-07-23_16:57:48.545.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Wiley
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-07 04:49:41	ID requested
⏵ 1	2024-07-23 16:57:49	announced

Dimet-Wiley AL, Latham CM, Brightwell CR, Neelakantan H, Keeble AR, Thomas NT, Noehren H, Fry CS, Watowich SJ, Nicotinamide N-methyltransferase inhibition mimics and boosts exercise-mediated improvements in muscle function in aged mice. Sci Rep, 14(1):15554(2024) [pubmed]

submitter keyword: exercise, gastrocnemius, nicotinamide N-methyltransferase inhibitor, 5A-1MQ, RT001, MS2, MS3

Stan Watowich
contact affiliation	Ridgeline Therapeutics
contact email	watowich@ridgelinetherapeutics.com
lab head
Andrea Wiley
contact affiliation	Ridgeline Therapeutics
contact email	wiley@ridgelinetherapeutics.com
dataset submitter

MassIVE dataset URI

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