PXD052062 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | E cadherin appears to be an important on/off switch for initiating bile canaliculi formation |
| Description | The mechanisms underlying cell polarization are fundamental in biology, yet they are not fully understood. This is particularly true for hepatocytes, which exhibit complex polarization distinct from other epithelial cells, enabling the formation of the bile canaliculi (BCs) network that is responsible for major liver function. To identify key proteins involved in hepatocytes polarization and BC formation, we performed a proteomic approach to compare the human hepatocyte cell line HepG2 to its sub clone HepG2/C3A which, shows much greater efficiency in forming mature BCs. We localized for the first time, LimA1 and Espin at the BC, which could play an important role at this place, and we confirmed the presence of NHE-RF1. Using a protein repression strategy directed against selected proteins, we highlighted the unique role of E cadherin in the initiation of BC formation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-09 |
| AnnouncementXML | Submission_2026-02-09_01:10:06.489.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD052062 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Hélène Diemer |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-05-07 02:42:30 | ID requested | |
| ⏵ 1 | 2026-02-09 01:10:07 | announced | |
Publication List
Keyword List
| submitter keyword: Human, bile canaliculi, shotgun proteomic, hepatocyte, Liver |
Contact List
| Sarah Cianférani |
|---|
| contact affiliation | Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg, France |
| contact email | sarah.cianferani@unistra.fr |
| lab head | |
| Hélène Diemer |
|---|
| contact affiliation | LSMBO |
| contact email | hdiemer@unistra.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD052062 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052062
- Label: PRIDE project
- Name: E cadherin appears to be an important on/off switch for initiating bile canaliculi formation
