PXD052018-2

PXD052018 is an original dataset announced via ProteomeXchange.

Title	Dissecting OGT’s TPR Domain to Identify Determinants of Cellular Function LC-MS/MS
Description	OGT’s tetratricopeptide(TPR) domain is important in substrate recognition, but there is little information on how changing the TPR domain impacts its cellular functions. Here, we investigate how altering OGT’s TPR domain impacts cell growth after the endogenous enzyme is deleted. We find that disrupting the TPR residues required for OGT dimerization leads to faster cell growth, whereas truncating the TPR domain slows cell growth.We also find that OGT requires eight of its 13 TPRs to sustain cell viability. OGT-8, like the nonviable shorter OGT variants, is mislocalized and has reduced Ser/Thr glycosylation activity; moreover, its interactions with most of wild-type OGT’s binding partners are broadly attenuated.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:17.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bettine Gibbs
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-06 07:33:48	ID requested
1	2024-05-06 07:58:44	announced
⏵ 2	2024-10-22 06:38:17	announced	2024-10-22: Updated project metadata.

10.1073/PNAS.2401729121;

submitter keyword: OGT, TPR, fibroblast,MEF

Suzanne Walker
contact affiliation	Department of Microbiology, Blavatnik Institue of Harvard Medical School
contact email	suzanne_walker@hms.harvard.edu
lab head
Bettine Gibbs
contact affiliation	Department of Microbiology, Blavatnik Instiute of Harvard Medical School
contact email	bgibbs@g.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD052018

PRIDE project URI

• PRIDE
  1. PXD052018
     1. Label: PRIDE project
     2. Name: Dissecting OGT’s TPR Domain to Identify Determinants of Cellular Function LC-MS/MS