PXD052015 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Docking a flexible basket onto the core of the nuclear pore complex |
Description | The nuclear basket attaches to the nucleoplasmic side of the nuclear pore complex (NPC), coupling transcription to mRNA quality control and export. The basket expands the functional repertoire of a subset of NPCs in S. cerevisiae by drawing a unique RNA/protein interactome. Yet, how the basket docks onto the NPC core remains unknown. By integrating AlphaFold-powered interaction screens, electron microscopy and membrane-templated reconstitution, we uncovered a membrane-anchored tripartite junction between basket and NPC core. The basket subunit Nup60 harbours three adjacent short linear motifs (SLiMs) which connect Mlp1, a parallel homodimer consisting of coiled-coil segments interrupted by flexible hinges, and the Nup85 subunit of the Y-complex. We reconstituted the Y-complex•Nup60•Mlp1 assembly on a synthetic membrane and validated the protein interfaces in vivo. Our study explains how a SLiM-based protein junction can substantially reshape NPC structure and function, advancing our understanding of compositional and conformational NPC heterogeneity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-11 |
AnnouncementXML | Submission_2024-07-11_04:37:55.727.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Markus Hartl |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-06 07:00:05 | ID requested | |
⏵ 1 | 2024-07-11 04:37:56 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Nup153,Nuclear pore complex, Mlp2, nuclear basket, Mlp1, membrane-based reconstitution, Y-complex, AlphaFold screen., nuclear envelope, SLiM, nucleocytoplasmic transport, NPC assembly, Tpr, Nup85, Nup60 |
Contact List
Alwin Köhler |
contact affiliation | Max Perutz Labs, University of Vienna and Medical University of Vienna, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9/3, 1030 Vienna, Austria |
contact email | alwin.koehler@maxperutzlabs.ac.at |
lab head | |
Markus Hartl |
contact affiliation | Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria |
contact email | proteomics@maxperutzlabs.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052015
- Label: PRIDE project
- Name: Docking a flexible basket onto the core of the nuclear pore complex