PXD051991-1

PXD051991 is an original dataset announced via ProteomeXchange.

Title	Characterization of marine cyanobacterium Synechococcus (WH8102 and WH5701) exoproteome under P-stress
Description	Proteins secreted by marine cyanobacterium Synechococcus under phosphorus stress is largely uncharacterized. This dataset characterizes the exoproteins for both an open ocean (WH8102) and coastal (WH5701) Synechococcus strain and were collected as part of the study "Dissolved organic phosphorus bond-class utilization by Synechococcus". Study Abstract: Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
HostingRepository	PRIDE
AnnounceDate	2024-07-15
AnnouncementXML	Submission_2024-07-14_22:24:42.986.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051991
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emily Waggoner
SpeciesList	scientific name: Synechococcus; NCBI TaxID: 1129;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-04 20:44:36	ID requested
⏵ 1	2024-07-14 22:24:43	announced

10.1093/FEMSEC/FIAE099;

10.6019/PXD051991;

submitter keyword: phosphate stress,cyanobacteria, exoproteome

Solange Duhamel
contact affiliation	Associate Professor, Molecular and Cellular Biology Department, University of Arizona
contact email	duhamel@arizona.edu
lab head
Emily Waggoner
contact affiliation	Molecular and Cellular Biology, University of Arizona
contact email	ewaggoner@arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051991
     1. Label: PRIDE project
     2. Name: Characterization of marine cyanobacterium Synechococcus (WH8102 and WH5701) exoproteome under P-stress