PXD051981 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | KDR-dependent tyrosine phosphorylation in HTLV-1 transformed cells |
Description | Human T-cell leukemia virus type 1 (HTLV-1) is linked to the development of adult T-cell leukemia (ATL) and the neuroinflammatory disease, HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The HTLV-1 Tax oncoprotein regulates viral gene expression and the NF-kB pathway to promote the survival of HTLV-1 infected T cells. In thsi study, we utilize a kinome-wide shRNA screen to identify the tyrosine kinase KDR/VEGFR2 as an essential survival factor of HTLV-1-transformed T cells. Inhibition of KDR induces apoptosis of Tax expressing HTLV-1-transformed cell lines and CD4+ T cells from HAM/TSP patients. Phosphoproteomics analysis of HTLV-1 transformed cells treated with a KDR inhibitor revealed inhibition of the phosphorylation of multiple receptors/cell surface proteins, ubiquitin conjugating systems, proteases, phosphatases, apoptotic regulatory factors, adhesion/extracellular matrix proteins and viral proteins. This work suggests that HTLV-1 Tax has hijacked KDR kinase activity to promote Tax stability and the proliferation and survival of HTLV-1 infected cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-14 |
AnnouncementXML | Submission_2024-06-14_03:04:03.362.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Edward Harhaj |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-03 17:30:47 | ID requested | |
⏵ 1 | 2024-06-14 03:04:03 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Tax,KDR, VEGFR2, HTLV-1 |
Contact List
Edward Harhaj |
contact affiliation | Penn State College of Medicine |
contact email | ewh110@psu.edu |
lab head | |
Edward Harhaj |
contact affiliation | Penn State College of Medicine |
contact email | ewh110@psu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051981
- Label: PRIDE project
- Name: KDR-dependent tyrosine phosphorylation in HTLV-1 transformed cells