PXD051976-1

PXD051976 is an original dataset announced via ProteomeXchange.

Title	Functional plasticity of HCO3- uptake and CO2 fixation in Cupriavidus necator H16
Description	Uptake and fixation of CO2 are central to strategies for CO2-based biomanufacturing. Cupriavidus necator H16 has emerged as a promising industrial host for this purpose. Despite its prominence, the ability to engineer C. necator inorganic carbon uptake and fixation is underexplored. Here, we test the role of endogenous and heterologous genes on C. necator inorganic carbon metabolism. Deletion of one of the four carbonic anhydrases in C. necator, β-carbonic anhydrase can, had the most deleterious effect on C. necator autotrophic growth. Replacement of this native uptake system with several classes of dissolved inorganic carbon (DIC) transporters from Cyanobacteria and chemolithoautotrophic bacteria recovered autotrophic growth and supported higher cell densities compared to wild-type (WT) C. necator in saturating CO2 in batch culture. Several heterologous strains with Halothiobacillus neopolitanus DAB2 (hnDAB2) expressed from the chromosome in combination with diverse rubisco homologs grew in CO2 equally or better than the wild-type strain. Our experiments suggest that the primary role of Can carbonic anhydrase during autotrophic growth is for bicarbonate accumulation to support anaplerotic metabolism, and an array of DIC transporters can complement this function. This work demonstrates flexibility in HCO3- uptake and CO2 fixation in C. necator, providing new pathways for CO2-based biomanufacturing.
HostingRepository	PRIDE
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_11:02:50.984.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Cupriavidus necator; NCBI TaxID: NEWT:106590;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 12:18:33	ID requested
⏵ 1	2026-01-23 11:02:53	announced

Panich J, Toppari E, Tejedor-Sanz S, Fong B, Dugan E, Chen Y, Petzold CJ, Zhao Z, Yoshikuni Y, Savage DF, Singer SW, fixation in Cupriavidus necator H16. Bioresour Technol, 410():131214(2024) [pubmed]

10.1016/j.biortech.2024.131214;

submitter keyword: gas fermentation, DAB, rubisco, carbonic anhydrase,C1 metabolism, CO2 biomanufacturing

Christopher J.Petzold
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051976
     1. Label: PRIDE project
     2. Name: Functional plasticity of HCO3- uptake and CO2 fixation in Cupriavidus necator H16