PXD051969-1

PXD051969 is an original dataset announced via ProteomeXchange.

Title	Etomoxir: A Promiscuous Fatty Acid Mimetic Chemoproteomic Probe
Description	Etomoxir has been used for decades as a popular small molecule inhibitor of Carnitine Palmitoyltransferase I, Cpt1, to block mitochondrial fatty acid b-oxidation. To test the specificity of etomoxir, we generated click chemistry enabled reagents to label etomoxir binding proteins in situ. Etomoxir bound to Cpt1, but also bound to a large array of diverse proteins that metabolize and transport fatty acids in the cytoplasm, peroxisome and mitochondria. Many of the most abundant proteins identified in primary hepatocytes were peroxisomal. The loss of Pex5, required for the import of peroxisomal matrix proteins, eliminated many of these proteins. By utilizing the promiscuous, covalent and fatty acid mimetic properties of etomoxir, novel etomoxir targets of fatty acid w-oxidation were revealed following the loss of Pex5. These data demonstrate that etomoxir is not specific for Cpt1 and is not appropriate as a tool to distinguish the biological effects of fatty acid oxidation.
HostingRepository	PRIDE
AnnounceDate	2024-05-04
AnnouncementXML	Submission_2024-05-04_11:50:36.724.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051969
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Wolfgang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 07:31:50	ID requested
⏵ 1	2024-05-04 11:50:37	announced
2	2024-10-22 06:38:11	announced	2024-10-22: Updated project metadata.

10.6019/PXD051969;

submitter keyword: Liver, Fatty Acid Oxidation, Cpt1, Etomoxir

Michael J. Wolfgang
contact affiliation	Johns Hopkins University School of Medicine
contact email	mwolfga1@jhmi.edu
lab head
Michael Wolfgang
contact affiliation	Johns Hopkins University School of Medicine
contact email	mwolfga1@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051969
     1. Label: PRIDE project
     2. Name: Etomoxir: A Promiscuous Fatty Acid Mimetic Chemoproteomic Probe