PXD051960-1

PXD051960 is an original dataset announced via ProteomeXchange.

Title	A proteomics resource for human iPSC-derived endothelial cells (iEC) - human FOXF2 KO versus WT control
Description	FOXF2, a major risk gene for stroke and SVD, the mechanisms of which are insufficiently understood. CRISPR/Cas9-mediated knockout of FOXF2 induced key features of BBB dysfunction, including compromised cell junction integrity, decreased transendothelial electrical resistance and dysregulated caveolae formation. Proteomic analysis of human iPSC derived endothelial cells revealed an impairment of pathways related to endocytosis and cell junctions, confirming a role of FOXF2 in BBB function. The observed disease features phenocopy those seen in an endothelial cell-specific mouse model of Foxf2 deficiency that we have developed in parallel, validating the relevance of our human BBB model to investigate neurovascular disorders.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_09:18:31.240.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 04:49:51	ID requested
⏵ 1	2025-09-02 09:18:31	announced

Dataset with its publication pending

submitter keyword: small vessel disease,FOXF2, iPSC, endothelial cells

Stefan F.
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD051960

PRIDE project URI

• PRIDE
  1. PXD051960
     1. Label: PRIDE project
     2. Name: A proteomics resource for human iPSC-derived endothelial cells (iEC) - human FOXF2 KO versus WT control