PXD051959-1

PXD051959 is an original dataset announced via ProteomeXchange.

Title	A proteomics resource for human iPSC-derived endothelial cells (iEC), smooth muscle cells (iSMC), pericytes (iPC) and astrocytes (iAS) in comparison to iPSCs and human primary cells - technical replicates
Description	Function and integrity of the blood-brain-barrier (BBB) is crucial for brain homeostasis, and its malfunction critically contributes to neurovascular and neurodegenerative disorders, including cerebral small vessel disease (SVD), a common cause of stroke and vascular dementia. So far, mechanistic studies on BBB function have been mostly conducted in mice and non-physiological in vitro models, which recapitulate disease features, but often lack complex phenotypes, have limited translatability to humans, and pose challenges for drug discovery. Therefore, we aimed to establish a fully iPSC-derived 3D model of the human blood-brain-barrier. To characterize and validate our somatic cell differentiation protocols we compared our iPSC-derived cells with commercially available human primary cells: brain microvascular endothelial cells (pEC), human umbilical vein endothelial cells (HUVEC), brain vascular pericytes (pPC), brain vascular smooth muscle cells (pSMC) and midbrain astrocytes (pAS).
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-08_13:31:01.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 04:45:38	ID requested
⏵ 1	2025-09-08 13:31:01	announced

Dataset with its publication pending

submitter keyword: Human, astrocytes, iPSC, pericytes, endothelial cells, smooth muscle cells

Stefan F.
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD051959

PRIDE project URI

• PRIDE
  1. PXD051959
     1. Label: PRIDE project
     2. Name: A proteomics resource for human iPSC-derived endothelial cells (iEC), smooth muscle cells (iSMC), pericytes (iPC) and astrocytes (iAS) in comparison to iPSCs and human primary cells - technical replicates