PXD051913 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Coimmunoprecipitation and following mass spectrometry analysis of EhRacJ |
Description | To find out the binding partners of EhRacJ, crosslinking and coimmunoprecipitation were performed. Then, eluted samples were submitted to Mass Spectrometry and Proteomics Core Facility, Johns Hopkins University School of Medicine and analyzed by mass spctrometry (shotgun proteomics). The coimmunoprecipitation and following analysis were conducted only a single time. You can find data of HA-EhRacJ from "2023_3_13_28_AM_FileSize_1261863517_Byte_F012933.mzid.gz", "2023_3_13_28_AM_FileSize_1261863517_Byte_F012933.mzid_2023_3_13_28_AM_File_Size__1261863517__Byte__F012933.MGF", "JS-E480-CS_230524_NozakiT_MS_DDA_S1_10pct.msf", "JS-E480-CS_230524_NozakiT_MS_DDA_S1_10pct.mzML", and "JS-E480-CS_230524_NozakiT_MS_DDA_S1_10pct.RAW". You can find data of pEhEx-HA (mock) from "2023_6_54_27_PM_FileSize_1168419053_Byte_F012936.mzid.gz", "2023_6_54_27_PM_File Size_1168419053_Byte_F012936.mzid_2023_6_54_27_PM_File_Size__1168419053__Byte__F012936.MGF", "JS-E480-CS_230524_NozakiT_MS_DDA_S4_10pct.msf", "JS-E480-CS_230524_NozakiT_MS_DDA_S4_10pct.mzML", and "JS-E480-CS_230524_NozakiT_MS_DDA_S4_10pct.RAW". |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-20 |
AnnouncementXML | Submission_2024-06-20_07:38:10.763.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD051913 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Misato Shimoyama |
SpeciesList | scientific name: Entamoeba histolytica HM-1:IMSS; NCBI TaxID: 294381; |
ModificationList | monohydroxylated residue; formylated residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-01 00:47:15 | ID requested | |
⏵ 1 | 2024-06-20 07:38:11 | announced | |
Publication List
Keyword List
submitter keyword: Entamoeba histolytica,coimmunoprecipitation, mass spectrometry analysis |
Contact List
Tomoyoshi Nozaki |
contact affiliation | Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo |
contact email | nozaki@m.u-tokyo.ac.jp |
lab head | |
Misato Shimoyama |
contact affiliation | The University of Tokyo |
contact email | shimoyama-misato142@g.ecc.u-tokyo.ac.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051913
- Label: PRIDE project
- Name: Coimmunoprecipitation and following mass spectrometry analysis of EhRacJ