PXD051896-1

PXD051896 is an original dataset announced via ProteomeXchange.

Title	Primary sclerosing cholangitis-specific inflammatory bowel disease phenotype is driven by an altered immune system shaped by circulatory markers.
Description	Approximately 80% of patients with Primary Sclerosing Cholangitis (PSC) also have an underlying Inflammatory Bowel Disease (IBD). Notably, PSC-IBD presents a unique phenotype compared to Ulcerative Colitis or Crohn’s Disease alone , which may increase the risk of colitis-associated neoplasia. This case-control study comprises a cohort of 15 patients in the PSC-IBD group, 30 patients in the IBD-alone group, and 19 patients in the control group. Through the analysis of patient serum and colon tissue proteomics, colon gene expression, fecal gut microbiota, and in vitro data with human monocytes, we identified a specific interplay between systemic circulation and gut microbiota dysbiosis that may predispose PSC-IBD patients to neoplasia development. Our study revealed that PSC-IBD patients show a shift in gut microbiota towards an increase in Intestinibacter, a bacterium known to exacerbate IBD conditions.Interestingly, when comparing the PSC-IBD group to the IBD-alone group, we observed elevated miR-21 expression levels in fasting serum and stool samples. Finally, we partially reproduce the inflammatory PSC-IBD profile using miR-21 and bile acid GCDCA stimulus in human-derived monocytes. Our findings suggest that circulating miR-21, along with bile acid GCDCA, tissue macrophage recruitment, and distinct microbiota profiles, may contribute to the unique phenotype of IBD in PSC patients.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_22:12:26.985.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Roudnický
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 10:29:54	ID requested
⏵ 1	2025-08-03 22:12:27	announced

Santos AA, Pires D, Marques V, Alesina N, Herraez E, Roudnick, ý P, Rodrigues PM, Godinho-Santos A, Bravo AC, Gouveia C, Saraiva S, Correia L, Crespo R, da Silva JP, Cravo M, Potesil D, Zdr, á, hal Z, Banales JM, Marin JJG, Torres J, Rodrigues CMP, Primary bile acid shapes peripheral immunity in inflammatory bowel disease-associated primary sclerosing cholangitis. Clin Sci (Lond), 138(12):703-716(2025) [pubmed]

10.1042/cs20256078;

submitter keyword: Human, proteomics, Primary Sclerosing Cholangitis, Exploris 480, Inflammatory Bowel Disease

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Pavel Roudnický
contact affiliation	Masaryk University
contact email	p.roudnicky@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD051896

PRIDE project URI

• PRIDE
  1. PXD051896
     1. Label: PRIDE project
     2. Name: Primary sclerosing cholangitis-specific inflammatory bowel disease phenotype is driven by an altered immune system shaped by circulatory markers.