PXD051892-2

PXD051892 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Datasets for Mec1/ATR signaling in Checkpoint-deficient (rad53Δ) cells
Description	The Mec1/ATR kinase is crucial for genome stability, yet the mechanism by which it prevents gross chromosomal rearrangements (GCRs) remains unknown. Here we find that in cells with deficient Mec1 signaling, GCRs accumulate due to the deregulation of multiple steps in homologous recombination (HR). Mec1 primarily suppresses GCRs through its role in activating the canonical checkpoint kinase Rad53, which ensures the proper control of DNA end resection. Upon loss of Rad53 signaling and resection control, Mec1 becomes hyperactivated and triggers a salvage pathway in which the Sgs1 helicase is recruited to sites of DNA lesions via the 911-Dpb11 scaffolds and phosphorylated by Mec1 to favor heteroduplex rejection and limit HR-driven GCR accumulation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:54.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bokun Xie
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C; 6x(13)C; phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 08:46:44	ID requested
1	2024-10-07 13:53:08	announced
⏵ 2	2024-10-22 06:43:54	announced	2024-10-22: Updated project metadata.

10.1038/S44318-024-00139-9;

submitter keyword: Mec1/ATR,Saccharomyces cerevisiae (baker's yeast), DNA damage checkpoint

Marcus Smolka
contact affiliation	Cornell University
contact email	mbs266@cornell.edu
lab head
Bokun Xie
contact affiliation	Graduate student
contact email	bx58@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD051892

PRIDE project URI

• PRIDE
  1. PXD051892
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Datasets for Mec1/ATR signaling in Checkpoint-deficient (rad53Δ) cells