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PXD051892-2

PXD051892 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomic Datasets for Mec1/ATR signaling in Checkpoint-deficient (rad53Δ) cells
DescriptionThe Mec1/ATR kinase is crucial for genome stability, yet the mechanism by which it prevents gross chromosomal rearrangements (GCRs) remains unknown. Here we find that in cells with deficient Mec1 signaling, GCRs accumulate due to the deregulation of multiple steps in homologous recombination (HR). Mec1 primarily suppresses GCRs through its role in activating the canonical checkpoint kinase Rad53, which ensures the proper control of DNA end resection. Upon loss of Rad53 signaling and resection control, Mec1 becomes hyperactivated and triggers a salvage pathway in which the Sgs1 helicase is recruited to sites of DNA lesions via the 911-Dpb11 scaffolds and phosphorylated by Mec1 to favor heteroduplex rejection and limit HR-driven GCR accumulation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:43:54.125.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBokun Xie
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList6x(13)C; 6x(13)C; phosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-30 08:46:44ID requested
12024-10-07 13:53:08announced
22024-10-22 06:43:54announced2024-10-22: Updated project metadata.
Publication List
10.1038/S44318-024-00139-9;
Keyword List
submitter keyword: Mec1/ATR,Saccharomyces cerevisiae (baker's yeast), DNA damage checkpoint
Contact List
Marcus Smolka
contact affiliationCornell University
contact emailmbs266@cornell.edu
lab head
Bokun Xie
contact affiliationGraduate student
contact emailbx58@cornell.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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