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PXD051883-1

PXD051883 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain
DescriptionHistone post-translational modifications play pivotal roles in eukaryotic gene expression. To date, most studies have focused on modifications in unstructured histone N-terminal tail domains and their binding proteins. However, transcriptional regulation by chromatin-effector proteins that directly recognize modifications in histone globular domains has yet to be clearly demonstrated, despite the richness of their multiple modifications. Here, we show that the ATP-dependent chromatin-remodeling BAF complex stimulates p53-dependent transcription through direct interaction with H3K56ac located on the lateral surface of the histone globular domain. Mechanistically, the BAF complex recognizes nucleosomal H3K56ac via the DPF domain in the DPF2 subunit and exhibits enhanced nucleosome-remodeling activity in the presence of H3K56ac. We further demonstrate that a defect in H3K56ac–BAF complex interaction leads to impaired p53-dependent gene expression and DNA damage responses. Our study provides direct evidence that histone globular domain modifications participate in the regulation of gene expression.
HostingRepositoryjPOST
AnnounceDate2024-09-30
AnnouncementXMLSubmission_2024-09-30_00:32:59.233.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJihyun Kim
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; N6-acetyl-L-lysine
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-30 03:51:23ID requested
12024-09-30 00:32:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: H3K56ac nucleosome
Histone globular domain
BAF complex
Contact List
Jaehoon Kim
lab head
Jihyun Kim
contact affiliationDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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