PXD051851-1

PXD051851 is an original dataset announced via ProteomeXchange.

Title	o The CutRS two-component system controls the Streptomyces secretion stress response by monitoring extracellular disulphide bond formation
Description	o Translocating unfolded proteins across cell membranes is essential in all domains of life and requires the conserved Sec machinery. In bacteria, Sec substrates fold on the extracellular face of the cytoplasmic membrane and misfolding triggers a stress response that involves production of HtrA-family proteins that act as dual function chaperone-proteases. In monoderm bacteria such as Streptomyces species, Sec substrates are translocated directly into the oxidising environment and typically contain even numbers of cysteine residues that form disulphide bonds to help the proteins fold. The Streptomyces secretion stress response is mediated by conserved two-component systems CssRS and CutRS and here we provide evidence that CutS senses disulphide bond formation via two conserved cysteine residues in its extracellular sensor domain. Breaking this disulphide bond activates CutRS and modulates the production of the quality control chaperones HtrA3 and HtrB. To our knowledge this represents a unique extracellular a sensing mechanism in bacteria.
HostingRepository	PRIDE
AnnounceDate	2025-08-09
AnnouncementXML	Submission_2025-08-09_09:42:01.590.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051851
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Streptomyces venezuelae; NCBI TaxID: 54571;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 10:37:29	ID requested
⏵ 1	2025-08-09 09:42:01	announced

10.6019/PXD051851;

submitter keyword: proteomics, Two component systems, cutRS,Streptomyces, Secretion stress, Regulation

Matt Hutchings
contact affiliation	Department Molecular Microbiology John Innes Centre Norwich Research Park Norwich, UK
contact email	matt.hutchings@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD051851

PRIDE project URI

• PRIDE
  1. PXD051851
     1. Label: PRIDE project
     2. Name: o The CutRS two-component system controls the Streptomyces secretion stress response by monitoring extracellular disulphide bond formation