PXD051838-1

PXD051838 is an original dataset announced via ProteomeXchange.

Title	Mass-spectrometry analysis of brain endothelial cells (BECs) from Foxf2iECKO and Ctrl mice
Description	To identify the molecular and cellular pathways mediating the effects of Foxf2 in brain endothelial cells (BECs), we performed proteomic analysis of mouse BECs. For this, we applied our previously published BEC enrichment protocol using magnetic-activated cell sorting (MACS) combined with liquid chromatography-mass spectrometry (LC-MS/MS) based proteomics (Todorov-Völgyi et al., 2024) to 6 months old animals. Proteomic analysis of isolated BECs captured a total of 4750 proteins. Out of these, 320 and 434 proteins were significantly up-, and downregulated, respectively in Cdh5-CreERT2;Foxf2fl/fl (Foxf2iECKO) vs Foxf2fl/fl (Ctrl) mice. In GO enrichment analyses of significantly downregulated proteins we found ‘establishment of endothelial barrier’, ‘nitric oxide metabolic process’ and ‘positive regulation of angiogenesis’ to be among the most affected categories. Fold-change ranking of significantly altered proteins marked Tie2 as one of the most strongly downregulated proteins. Notably, several proteins involved in Tie2-regulated processes, including Nos3 and Ptgis (implicated in nitric oxide metabolic process), Rap1b, Tjp1, Cldn5, and Cdh5 (implicated in establishment of endothelial barrier), and Tie2, Eng, and Itgb1 (implicated in angiogenesis) were downregulated in BECs from Foxf2iECKO mice. Collectively, these findings demonstrate a critical role of endothelial Foxf2 in maintaining BBB integrity and Tie2 signaling.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_08:30:41.674.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 02:31:06	ID requested
⏵ 1	2025-09-02 08:30:42	announced

Dataset with its publication pending

submitter keyword: barin endothelial cells,FOXF2, TIE2, mouse

Stefan F.
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051838
     1. Label: PRIDE project
     2. Name: Mass-spectrometry analysis of brain endothelial cells (BECs) from Foxf2iECKO and Ctrl mice