PXD051818-1

PXD051818 is an original dataset announced via ProteomeXchange.

Title	Uterus-mimetic extracellular microenvironment for engineering female reproductive system
Description	Addressing the complexities and challenges inherent in endometrial biology and regeneration, this study develops a uterus-mimetic microenvironment to reconstitute the female reproductive system. Leveraging a decellularized uterus extracellular matrix (UEM), this approach supports development and functions of endometrial organoids (EOs) more effectively than the current gold standard, Matrigel. Extensive proteomic analysis across eight different tissue-derived matrices reveals that those closely mirroring the protein profile of UEM–especially those abundant in proteins specific to the female reproductive system–more effectively support EO growth. These proteins, particularly fibronectin and decorin, are identified as key enhancers of EO development, with decorin notably amplifying Wnt7a gene expression, a pathway important for endometrial development. In vivo, UEM demonstrates remarkable efficacy in regenerative applications, notably enhancing epithelial regeneration and increasing pregnancy rates in an endometrium injury model. Moreover, the sophisticated in vitro modeling provided by UEM facilitates effective decidualization of endometrial stromal cells and structural superiority when co-cultured with EOs, thus offering an enhanced platform for studying blastocyst implantation. Collectively, these findings demonstrate UEM's pivotal role in reconstituting reproductive system, highlighting its effectiveness in producing organoids that are not only structurally and functionally robust but also therapeutically potent.
HostingRepository	PRIDE
AnnounceDate	2024-05-08
AnnouncementXML	Submission_2024-05-08_10:16:08.266.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Sun Choi
SpeciesList	scientific name: Sus scrofa (Pig); NCBI TaxID: 9823;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-28 04:01:28	ID requested
⏵ 1	2024-05-08 10:16:08	announced

Dataset with its publication pending

submitter keyword: LC-MSMS,Porcine, Extracellular matrix

Seung-Woo Cho
contact affiliation	Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea
contact email	seungwoocho@yonsei.ac.kr
lab head
Yi Sun Choi
contact affiliation	Yonsei University
contact email	yisunchoi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD051818

PRIDE project URI

• PRIDE
  1. PXD051818
     1. Label: PRIDE project
     2. Name: Uterus-mimetic extracellular microenvironment for engineering female reproductive system