PXD051801-1

PXD051801 is an original dataset announced via ProteomeXchange.

Title	Decoding the human adrenal cortex matrisome: the composition and regulation of ECM proteins of the outer and inner microenvironment.
Description	The extracellular matrix (ECM) comprises macromolecules that shape a complex three-dimensional network. Filling the intercellular space and playing a crucial role in the structure and function of tissues, ECM regulates essential cellular processes such as adhesion, differentiation, and cell signaling. In the human adrenal gland, composed of cortex and medulla surrounded by a capsule, the ECM has not yet been directly described, although its impact on the processes of proliferation and steroidogenesis of the adrenal cortex is recognized. This study proposes a comprehensive analysis aims to comprehensively analyze the ECM of the adult human adrenal cortex, which is separated into outer fraction (OF) and inner fraction (IF), by comparing their proteomic profiles. The study discusses the composition, spatial distribution, and relevance of differentially expressed ECM signatures of the adrenal cortex matrisome on adrenal structure and function. The findings were validated through database analysis (cross-validation), histochemical, and immunohistochemical approaches. A total of 121 ECM proteins were identified and categorized into glycoproteins, collagens, ECM regulators, proteoglycans, ECM-affiliated proteins, and secreted factors. Thirty-one ECM proteins were identified only in OF, 9 only in IF, and 81 were identified in common with both fractions. Additionally, 106 ECM proteins were cross-validated using Human matrisome DB 2.0 and the proteins differentially expressed in OF and IF, were identified. This study provides significant insights into the composition and regulation of the ECM in the human adrenal cortex, shedding light on the adrenal microenvironment and its role in the functioning, maintenance, and renewal of the adrenal gland.
HostingRepository	PRIDE
AnnounceDate	2024-09-11
AnnouncementXML	Submission_2024-09-11_10:29:32.616.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051801
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jean Lucas Kremer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-27 14:54:27	ID requested
⏵ 1	2024-09-11 10:29:33	announced
2	2024-10-22 06:58:00	announced	2024-10-22: Updated project metadata.

10.1016/J.MBPLUS.2024.100158;

10.6019/PXD051801;

submitter keyword: human adrenal gland,Extracellular matrix, cortex, matrisome.

Jean Lucas Kremer
contact affiliation	Laboratory of Cellular Structure and Function, Department of Anatomy; Institute of Biomedical Science, University of São Paulo, São Paulo,Brazil.
contact email	Jl.kremer@usp.br
lab head
Jean Lucas Kremer
contact affiliation	Laboratory of Cellular Structure and Function, Department of Anatomy; Institute of Biomedical Science, University of São Paulo
contact email	jl.kremer@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051801
     1. Label: PRIDE project
     2. Name: Decoding the human adrenal cortex matrisome: the composition and regulation of ECM proteins of the outer and inner microenvironment.