PXD051785 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Rules of engagement for condensins and cohesins guide mitotic chromosome formation. |
| Description | During mitosis, interphase chromatin is rapidly converted into rod-shaped mitotic chromosomes. Using Hi-C, imaging, proteomics and polymer modeling, we determine how the activity and interplay between loop-extruding SMC motors accomplishes this dramatic transition. Our work reveals rules of engagement for SMC complexes that are critical for allowing cells to refold interphase chromatin into mitotic chromosomes. We find that condensin disassembles interphase chromatin loop organization by evicting or displacing extrusive cohesin. In contrast, condensin bypasses cohesive cohesins, thereby maintaining sister chromatid cohesion while separating the sisters. Studies of mitotic chromosomes formed by cohesin, condensin II and condensin I alone or in combination allow us to develop new models of mitotic chromosome conformation. In these models, loops are consecutive and not overlapping, implying that condensins do not freely pass one another but stall upon encountering each other. The dynamics of Hi-C interactions and chromosome morphology reveal that during prophase loops are extruded in vivo at ~1-3 kb/sec by condensins as they form a disordered discontinuous helical scaffold within individual chromatids. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-16 |
| AnnouncementXML | Submission_2025-12-16_13:45:19.905.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kumiko Samejima |
| SpeciesList | scientific name: Gallus gallus (Chicken); NCBI TaxID: NEWT:9031; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-04-26 12:32:03 | ID requested | |
| ⏵ 1 | 2025-12-16 13:45:20 | announced | |
Publication List
Keyword List
| submitter keyword: condensins, SLIAC, DIA,Mitosis, cohesins, chromosomes |
Contact List
| Bill Earnshaw |
|---|
| contact affiliation | University of Edinburgh |
| contact email | bill.earnshaw@ed.ac.uk |
| lab head | |
| Kumiko Samejima |
|---|
| contact affiliation | University of Edinburgh |
| contact email | kumiko.samejima@ed.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD051785 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051785
- Label: PRIDE project
- Name: Rules of engagement for condensins and cohesins guide mitotic chromosome formation.
